Phytochemical Analysis And In Vitro Antimicrobial Activity Of Piliostigma Thoningii Roots, Leaves And Stem Bark Extracts On Escherichia Coli Isolated From Wounds Of Cattle
- Author
- Chigogo, Panashe
- Title
- Phytochemical Analysis And In Vitro Antimicrobial Activity Of Piliostigma Thoningii Roots, Leaves And Stem Bark Extracts On Escherichia Coli Isolated From Wounds Of Cattle
- Abstract
-
The high cost of antibiotics in the treatment of livestock diseases is one of the problems in Zimbabwe. Studies have shown that medicinal plants can been used as an alternative form of treatment. The aim was to investigate the chemical composition of Piliostigma thonningii roots, leaf and bark extracts against bovine Escherichia coli isolated from infected wounds of cattle. It was hypothesized that P. thonningi have antimicrobial effects against E. coli. Plant samples were taken from different areas in Gokwe district. Bacteriological methods were used to isolate and identify E. coli. Aqueous and methanolic extracts of concentrations 25, 50 and 75mg/ml were prepared and their antibacterial activity were tested against E. coli using the disc diffusion method on MHA. Amoxycillin was used as a positive control and methanol and aqueous were used as negative controls. Zones of inhibition were measured in mm and the MIC was determined. Both aqueous and methanolic of bark, root and leaf extracts exhibited antibacterial activity against the isolates and statistical analysis showed an increase in inhibition zone with increasing concentration. The highest was methanolic extracts, bark 14.89 ± 0.167, roots 10.97± 0.645 and leaves 9.46 ± 0.951, irrespectively. ANOVA showed a statistical difference among the Zone Of Inhibition or the extracts and concentrations (p<0.05). The investigation indicated that P. thonningi extracts have the therapeutical potential. Ferric Chloride (FeCl) test was used to test for phenols, while the Akter’s method was used to test for flavonoids. Tannis were screened using Shukla’s method test and alkaloids were tested using Wagner’s reagent. It is recommended that further studies must be conducted to deepen understanding of P. thonningii's pharmacological properties and therapeutic potential.
- Date
- JUNE 2024
- Publisher
- BUSE
- Keywords
- Phytochemical, Vitro Antimicrobial, Piliostigma Thoningii, Escherichia Coli,Wounds Of Cattle
- Supervisor
- Mr J Ndava
- Item sets
- Department of Biological Sciences
