Qualitative And Quantitative Phytochemical Analysis Of Four Plants Used To Treat Theileriosis In Cattle. · Electronic Theses and Dissertations

Author: Madzinga Juliana
Title: Qualitative And Quantitative Phytochemical Analysis Of Four Plants Used To Treat Theileriosis In Cattle.
Abstract: ABSTRACT
Extracts of various parts of Erythina abbssinicia, Cissus quadrangularis, Cassia abbreviata and Brachylaena rotundata are claimed to have potent bioactivity. Traditional practice and use of the plants is based on guesswork and experience. In this research the phytochemical composition of hydroethanolic stem and bark extracts of the plants was evaluated. Maceration of powdered plant material in 60 % ethanol was employed to extract phytochemicals. Presence of alkaloids was confirmed using Mayer’s test, foaming test was applied for sapponins. To test for phenolics, flavonoids, glycosides and tannins, lead acetate, alkaline, Bontager and the ferric chloride tests were used respectively. The acid test was done for quinones. To test for terpenoids and steroids, chloroform/sulphuric acid and alcohol/sulphuric acid test were used respectively. Three phytochemicals namely tannins, phenolics and alkaloids were quantified. The dry extrant yields obtained for each plant are as follows: C. abbreviata 19.9 %, B. rotundata 16.6 %, C. quadrangularis 15.5 % and lastly E. abbyssinica 13.8 %. All the plants had positive results for the tested phyto-compoundas except C. abbreviata which tested negative for saponins and steroids. In quantitative phytochemical analysis, C. quadrangularis had the highest content of tannins (476 mg/mL), followed by E. abbyssinica (400 mg/mL), C. abbreviata (180 mg/mL) and B. rotundata (123 mg/mL). The findings of this research provide an insight into the phytochemical composition of E. abbyssinica, C. quadrangularis, C. abbreviata and B. rotundata providing a baseline for further exploration of the plants’ phytochemicals in managing livestock diseases.
Date: JUNE 2024
Publisher: BUSE
Keywords: -Erythina abbssinicia
-Cissus quadrangularis
-Cassia abbreviata
-Brachylaena rotundata
Supervisor: NIL

Item sets: Department of Animal Sciences

Media: Juliana Madzinga