Evaluation of antifungal activity of tannins isolated from Thespesia Garckeana bark extract · Electronic Theses and Dissertations

Author: Vushe, Fortune M.
Title: Evaluation of antifungal activity of tannins isolated from Thespesia Garckeana bark extract
Abstract: The study aimed to extract and characterize tannins from the bark of Thespesia garckeana and evaluate their antifungal activity against Candida albicans and Candida endophytes. Tannins were extracted using 90% ethanol and isolated using thin layer chromatography (TLC). Qualitative tests confirmed the presence of tannins, including ferric chloride, vanillin, and gelatine tests. UV-vis spectroscopy analysis showed the highest absorbance peak at 425 nm. The antifungal activity of the isolated tannins was assessed using the agar well diffusion method at concentrations of 25%, 50%, and 100%. Miconazole was used as a positive control. The zones of inhibition were measured in milli-meters. For Candida albicans, the mean zones of inhibition were 3.33 mm (25% tannins), 11.00 mm (50% tannins), 17.33 mm (100% tannins), and 22.33 mm (10% miconazole). For Candida endophytes, the mean zones were 7.67 mm (25% tannins), 13.67 mm (50% tannins), 23.33 mm (100% tannins), and 24.00 mm (10% miconazole). The results demonstrated that tannins extracted from T. garckeana bark possess significant antifungal activity against both Candida species, with higher concentrations exhibiting stronger inhibition. The antifungal activity of 100% tannins was comparable to the positive control, 10% miconazole and the MIC value was observed to be 25% (0.25 ml/ml). These findings suggest that T. garckeana tannins could be a promising natural alternative for managing fungal infections.
Date: June 2024
Publisher: BUSE
Keywords: Tannins; Antifungal
Supervisor: Dr. L. Gwatidzo

Item sets: Department of Chemistry

Media: Vushe, Fortune M..pdf