Investigating the Antifungal activity of fungal Endophytes isolated from Typha Latifolia against Candida Albicans. · Electronic Theses and Dissertations

Author: Moyo, Donewell
Title: Investigating the Antifungal activity of fungal Endophytes isolated from Typha Latifolia against Candida Albicans.
Abstract: The research is aimed at extracting and isolating fungal endophytes from Typha Latifolia and evaluating their antifungal activity against Candida albicans. Endophytes were extracted from the roots, stem and leaves of the Typha plant and pure cultures were cultivated observing all aseptic techniques. The antifungal activity of the isolated endophytes was assessed using the disk diffusion Method. Miconazole was used as a positive control. The zones of inhibition were measured in milli-meters. The mean zones of inhibition were 0 mm for leave endophytes, 15.7 mm for stem endophytes, 15.7 mm for root endophytes, and 24.7 mm for 10% miconazole. The results demonstrated that fungal endophytes extracted from T. Latifolia possess significant antifungal activity against Candida albicans, with higher activity exhibited by the endophytes extracted from the roots and the stem. The antifungal activity of the roots and stem endophytes was comparable to the positive control, 10% miconazole. These results suggest that T.Latifolia fungal endophytes could be a promising natural alternative for managing fungal infections.
Date: June 2024
Publisher: BUSE
Keywords: fungal endophytes; Candida albicans
Supervisor: Dr Mugocheki