Quantification of anthocyanin pigments in acer PALMATUM 'ATROPURPUREUM'-UV-VIS Spectometry

Author
Banda, Mekeyani
Title
Quantification of anthocyanin pigments in acer
PALMATUM 'ATROPURPUREUM'-UV-VIS Spectometry
Abstract
The aim of this research is to develop a reliable UV-Vis spectroscopic method for the
quantitative determination of chlorophyll content in the leaves of Acer palmatum
'Atropurpureum', with the goal of gaining a deeper understanding of the factors that influence
the accumulation of these pigments in this plant species. The experimental design was done
and leaves of Acer palmatum 'Atropurpureum' were collected from a local nursery located in
Bindura, Mashonaland Central, Zimbabwe and the chronological sequences of the experiment
were followed. The results of the chlorophyll extraction and quantification demonstrate the
effectiveness of the UV-Vis spectroscopic method for determining the concentrations of
chlorophyll a and chlorophyll b in the Acer palmatum 'Atropurpureum' leaf samples.
Three different extraction solvents, acetone, ethanol, and dimethylformamide (DMF), were
evaluated, and the results showed that acetone was the most effective solvent for extracting
and quantifying the chlorophyll pigments. From this research, conclusion have been drawn
from tangible evidence and calibration curves were constructed for chlorophyll A and
chlorophyll B using standard solutions in the respective extraction solvents (acetone, ethanol,
and DMF). The absorbance values at the specific wavelengths (662 nm for chlorophyll A and
645 nm for chlorophyll B) were plotted against the known concentrations of the chlorophyll
standards. The total chlorophyll content in the Acer palmatum 'Atropurpureum' leaves ranged
from 2.37 mg/g dry weight (ethanol) to 2.55 mg/g dry weight (acetone), with the acetone
extract showing the highest chlorophyll content.
Date
2024
Publisher
BUSE
Keywords
spectroscopic
quantification
Supervisor
G Katengeza
Item sets
Department of Science and Mathematical Foundations
Media
Banda M.pdf

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